Modulatory Role of Reactive Oxygen Species in Root Development in Model Plant of Arabidopsis thaliana

Reactive oxygen species (ROS), a type of oxygen reduction products monoelectronic, has a high chemical activity of O2. Although ROS pose a potential risk to all organisms through induced oxidative stress, which is inseparable from the role of ROS in the development of the individual can not be ignored. Among them, the role of ROS in the model Arabidopsis thaliana plants deeply studied. Mounting evidence indicates that ROS is important for the development of roots and root hairs. In this review, we provide an updated perspective on the progress of the latest research related to the role of ROS in the regulation of the right of maintenance of stem roots and differentiation of cells, redox regulation of the cell cycle, and the initiation of the hair roots during root growth. Among the various types of ROS, O2 • – and H2O2 has been studied extensively, and they show a different gradient distribution in the roots. The concentration of O2 • – decreased along the gradient of the meristem into the transition zone and H2O2 concentration decreased along the gradient of differentiation zone to zone of elongation. This gradient is governed by the peroxidase, which is modulated by UPBEAT1 (UPB1) transcription factors. In addition, several transcription factors, such as app1, ABO8, PHB3, and RITF1, which is involved in signaling pathways brassinolide, gathered as a signal to regulate ROS roots stem cell maintenance. Furthermore, superoxide anion (O 2 • -) resulting from oxidation in mitochondria, ROS generated during metabolism plasmid, H2O2 is produced in apoplasts, and catalysis respiratory burst oxidase homolog (RBOH) in cell membranes. Furthermore, ROS can act as a signal to regulate the redox status, which regulate the expression of cell-cycle component CYC2, 3, CYCB1, 1, and associated proteins retinoblastoma-, thereby controlling the development of cell-cycle. At the root maturation zone, epidermal cells located at position H cells appeared to form hair cells, and plant hormones such as auxin and ethylene regulates the formation of hair follicles through ROS. In addition, the accumulation of ROS can affect hormone signal transduction and vice versa. Data on the relationship between nutrient stress and ROS signaling in the development of hair follicles is rare. However, the fact that ROBHC / RHD2 or RHD6 is specifically expressed in cells and hair follicles caused by nutrients, may explain the relationship. Future studies should focus on the mechanisms underlying the regulation of hair roots development through the interaction of ROS with signaling hormones and nutritional components.

SYPL1 Inhibits Apoptosis in Pancreatic Ductal Adenocarcinoma via Eradication of ROS-induced ERK activation

Synaptophysin-like 1 (SYPL1) is associated protein neuroendokrin-. SYPL1 role in pancreatic ductal adenocarcinoma (PDAC) and the underlying molecular mechanisms remain unclarified. Here, after analyzing five datasets (GSE15471, GSE16515, GSE28735, TCGA and PACA-AU) and 78 patients with PDAC from Sun Yat-sen University Cancer Center, we show that SYPL1 is regulated in PDAC and that high levels SYPL1 indicated poor prognosis , bioinformatics analysis implied that SYPL1 associated with cell proliferation and cell death. To validate these findings, gain-of-function and loss-of-function experiments were carried out, and we found that SYPL1 promoted cell proliferation in vitro and in vivo and that it protected the cells from apoptosis. Mechanistic studies revealed that extracellular-regulated protein kinase (ERK) activation is responsible for the ongoing cell death resulting from the knockdown of SYPL1. In addition, bioinformatics analysis showed that expression of SYPL1 positively correlated with antioxidant activity. Reactive oxygen species (ROS) were upregulated in cells with SYPL1 knockdown and vice versa. ROS-regulated lead to activation of ERK and cell death. These results indicate that SYPL1 plays an important role in PDAC and promote cancer cell survival by pressing the ROS-induced activation of ERK.

Modulatory Role of Reactive Oxygen Species in Root Development in Model Plant of Arabidopsis thaliana
Modulatory Role of Reactive Oxygen Species in Root Development in Model Plant of Arabidopsis thaliana

hemoglobin oxygenation state defines the dynamics of water molecules in the vicinity

This study focuses on assessing the potential impact of changes in hemoglobin (Hb) oxygenation in the state of water in the hydration shell as a contribution to the deformability of red blood cells. Microwave Dielectric Spectroscopy (MDS) is used to monitor changes in the interaction between water molecules and hemoglobin, the number of water molecules in a protein shell hydration, and water dynamics pre-proteins in response to a transition of Hb of the tense (T) to relaxed (R) countries, and otherwise. A measurement of hemoglobin solutions of different concentrations (5 g / dl-30 g / dl) phosphate-buffered saline buffer. Cole-cole main water relaxation peak parameters in terms of the interaction of water molecules (dipole-dipole / dipole ion) during oxygenation-deoxygenation cycle is used to analyze the data obtained. Mobility of water represented by α as a function of τ-ln differ dramatically between R (oxygen) state and T (deoxygenated) state Hb at physiologically relevant concentrations (30 g / dl-35 g / dl or 4.5 MM 5, 5 mM).

Rat IgG-FITC conjugate (isotype control) (Isotype control)

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EUR 196.8

Rat IgG-Biotin conjugate (isotype control) (Isotype control)

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Rat IgG Isotype Control

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Description: Purified Rat IgG Isotype Control

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PE Rat IgM, κ Isotype Control

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PE Rat IgM, κ Isotype Control

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PE Rat IgM, κ Isotype Control

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PE rat IgM, κ Isotype Control

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Description: Available in various conjugation types.

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EUR 933.6

Rat IgM Isotype Control

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Rat IgM Isotype Control

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Rat IgM Isotype Control

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Rat IgM Isotype Control

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IgM, Rat Isotype Control (PE)

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IgM, Rat Isotype Control (PE)

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Rat IgM Isotype Control PE

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Rat IgG Isotype Control

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Rat IgG Isotype Control

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PE Rat IgM, κ Isotype Control

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PE Rat IgM, κ Isotype Control

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PE Rat IgM, κ Isotype Control

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PE Rat IgM, κ Isotype Control

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PE Rat IgM, κ Isotype Control

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APC Rat IgM, κ Isotype Control

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APC Rat IgM, κ Isotype Control

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APC Rat IgM, κ Isotype Control

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PE Rat IgG1, κ Isotype Control

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PE Rat IgG1, κ Isotype Control

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PE Rat IgG1, κ Isotype Control

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PE Goat IgG Isotype Control

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Description: Available in various conjugation types.

PE rat IgG1, Isotype Control

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EUR 303.33
Description: Available in various conjugation types.

PE rat IgG1, Isotype Control

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EUR 715
Description: Available in various conjugation types.

PE rat IgG1, κ Isotype Control

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Description: Available in various conjugation types.

PE rat IgG1, κ Isotype Control

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Description: Available in various conjugation types.

Rat IgG1 Isotype Control

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Goat F(ab )2 IgG Isotype Control

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Goat F(ab )2 IgG Isotype Control

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Goat F(ab )2 IgG Isotype Control

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Rat IgG1 Isotype Control

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Goat IgG Isotype Control

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Rat IgG1 Isotype Control

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Goat F(ab )2 IgG Isotype Control

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Goat IgG Isotype Control

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Rat IgG1 Isotype Control

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Goat IgG Isotype Control

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Goat F(ab )2 IgG Isotype Control

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Goat F(ab )2 IgG Isotype Control

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Goat IgG Isotype Control

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Isotype Control, B-Z1, IgG1, PE

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EUR 312.32

Rat IgG1 Isotype Control

RIGG1A-100 100 µg
EUR 654.05

Rat IgG1 Isotype Control

RIGG1A-25 25 µg
EUR 322.08

Rat IgG1 Isotype Control

RIGG1F-50 50 µg
EUR 357.02

Rat IgG1 Isotype Control

RIGG1F-500 500 µg
EUR 1055.9

Rat IgG1 Isotype Control

RIGG1PE-100 100 µg
EUR 636.58

Rat IgG1 Isotype Control

RIGG1PE-25 25 µg
EUR 304.61

Rat IgG1 Isotype Control

RIGG1PP5.5-100 100 µg
EUR 706.46

Rat IgG1 Isotype Control

RIGG1PP5.5-25 25 µg
EUR 322.08

Rat IgG1 Isotype Control

RIGG1PU-50 50 µg
EUR 217.25

Rat IgG1 Isotype Control

RIGG1PU-500 500 µg
EUR 479.33

Rat IgG1 Isotype Control

abx405039-100g 100 µg
EUR 275

Rat IgG1 Isotype Control

abx405041-100g 100 µg
EUR 200

Rat IgG1 Isotype Control (PE)

abx405042-100g 100 µg
EUR 250

Goat F(ab')2 IgG Isotype Control

abx405076-250g 250 µg
EUR 300

Goat F(ab')2 IgG Isotype Control (PE)

abx405078-100g 100 µg
EUR 437.5

Goat IgG (H+L) isotype control

BA1044 1mg
EUR 15

IgG1, Rat Isotype Control

MBS655540-05mg 0.5mg
EUR 420

IgG1, Rat Isotype Control

MBS655540-5x05mg 5x0.5mg
EUR 1730

IgM, Rat Isotype Control (PE/CY5)

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EUR 505

IgM, Rat Isotype Control (PE/CY5)

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EUR 2125

IgG1, Rat Isotype Control (PE)

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IgG1, Rat Isotype Control (PE)

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IgM, Rat Isotype Control (APC)

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EUR 525

IgM, Rat Isotype Control (APC)

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EUR 2215

IgG, Goat Isotype Control

MBS655594-5mg 5mg
EUR 370

IgG, Goat Isotype Control

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EUR 1505

IgG, Goat Isotype Control

MBS655640-4mg 4mg
EUR 385

IgG, Goat Isotype Control

MBS655640-5x4mg 5x4mg
EUR 1590

IgG, Fc, Goat Isotype Control

MBS655649-2mg 2mg
EUR 425

IgG, Fc, Goat Isotype Control

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EUR 1770

IgG, Rat Isotype Control (APC)

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EUR 475

IgG, Rat Isotype Control (APC)

MBS655652-5x01mg 5x0.1mg
EUR 1975

IgM, Rat Isotype Control (PE/Cy7)

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EUR 465

IgM, Rat Isotype Control (PE/Cy7)

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EUR 1950

IgG, Rat Isotype Control (PE/Cy7)

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EUR 460

IgG, Rat Isotype Control (PE/Cy7)

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IgG, Rat Isotype Control (Cy5)

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EUR 485

IgG, Rat Isotype Control (Cy5)

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EUR 2025

IgG, Rat Isotype Control (PE/CY5)

MBS655734-01mg 0.1mg
EUR 460

IgG, Rat Isotype Control (PE/CY5)

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EUR 1925

Rat IgG1 Isotype Control PE

MBS697135-01mg 0.1mg
EUR 235

Rat IgG1 Isotype Control PE

MBS697135-5x01mg 5x0.1mg
EUR 1005

Goat IgG isotype control

MBS176681-1mg 1mg
EUR 115

Goat IgG isotype control

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EUR 150

Goat IgG isotype control

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EUR 525

Rat IgG1 Isotype Control

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EUR 410

Rat IgG1 Isotype Control

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EUR 1550

Goat IgG (isotype control), PE

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EUR 225

APC Rat IgM, κ Isotype Control

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EUR 165

APC Rat IgM, κ Isotype Control

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EUR 240

APC Rat IgM, κ Isotype Control

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EUR 60

APC Rat IgM, κ Isotype Control

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EUR 103.5

APC Rat IgM, κ Isotype Control

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EUR 45

PE Rat IgG1, κ Isotype Control

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PE Rat IgG1, κ Isotype Control

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PE Rat IgG1, κ Isotype Control

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PE Rat IgG1, κ Isotype Control

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EUR 75

PE Rat IgG1, κ Isotype Control

RD22499F-25ug 25μg
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Rat IgG1 Isotype Control

ab-131-428 500 µg
EUR 290

Mouse IgG2a Isotype Control Antibody:Biotin (HOPC-1) Mouse IgG2a Isotype Control Antibody:Biotin (HOPC-1)

MAB12223-100 0.1
EUR 196.47
Description: Mouse monoclonal antibody, IgG2a isotype control, conjugated to Biotin

Mouse IgM Isotype Control

abx139003-01mg 0.1 mg
EUR 376.8

Mouse IgM Isotype Control (PE)

abx139032-01mg 0.1 mg
EUR 510

Mouse IgM Isotype Control

abx020500-02mg 0.2 mg
EUR 526.8

Rat IgG2a Isotype Control

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EUR 57.2

Rat IgG2b Isotype Control

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Mouse IgM Isotype Control PE

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Rat IgG2a Isotype Control PE

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Rat IgG2a Isotype Control PE

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Rat IgG2b Isotype Control PE

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Rat IgG2b Isotype Control

31R-IC001 500 ug
EUR 195
Description: Purified Rat IgG2b Isotype Control

Mouse IgM Isotype Control

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EUR 376.8

Rat IgG2a Isotype Control

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EUR 577.2

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EUR 393.6

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Rat IgG2a Isotype Control

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EUR 360

Rat IgG2a Isotype Control

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EUR 460.8

Rat IgG2a Isotype Control

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EUR 678

Rat IgG2c Isotype Control

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EUR 644.4

Rat IgG1 Isotype Control (RPE)

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EUR 427.2

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EUR 460.8

Rat IgG2a Isotype Control

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  • 0.1 mg
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Rat IgG2b Isotype Control

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EUR 460.8

Rat IgG2b Isotype Control

20-abx405053
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  • 0.1 mg
  • 1 mg

Mouse IgM Isotype Control

abx405062-100tests 100 tests
EUR 493.2

Goat F(ab')2 IgG Isotype Control (RPE)

abx405078-05ml 0.5 ml
EUR 644.4

PE Mouse IgM, κ Isotype Control

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EUR 158

PE Mouse IgM, κ Isotype Control

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EUR 233

PE Mouse IgM, κ Isotype Control

DL22337F-25ug 25μg
EUR 137

PE Mouse IgM, κ Isotype Control

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EUR 182

FITC Rat IgM, κ Isotype Control

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EUR 158

FITC Rat IgM, κ Isotype Control

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EUR 233

FITC Rat IgM, κ Isotype Control

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EUR 137

FITC Rat IgM, κ Isotype Control

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EUR 182

APC Rat IgG1, κ Isotype Control

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EUR 170

APC Rat IgG1, κ Isotype Control

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EUR 245

APC Rat IgG1, κ Isotype Control

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EUR 147.5

APC Rat IgG1, κ Isotype Control

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EUR 197

PE Rat IgG2a, κ Isotype Control

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EUR 158

PE Rat IgG2a, κ Isotype Control

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EUR 233

PE Rat IgG2a, κ Isotype Control

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EUR 137

PE Rat IgG2a, κ Isotype Control

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EUR 182

PE Rat IgG2b, κ Isotype Control

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EUR 158

PE Rat IgG2b, κ Isotype Control

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EUR 233

PE Rat IgG2b, κ Isotype Control

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EUR 137

PE Rat IgG2b, κ Isotype Control

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EUR 182

PE mouse IgM Isotype Control

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EUR 235.2
Description: Available in various conjugation types.

PE mouse IgM Isotype Control

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EUR 336
Description: Available in various conjugation types.

APC rat IgG1, Isotype Control

E16FRCA001-025U 25 μg
EUR 325
Description: Available in various conjugation types.

APC rat IgG1, Isotype Control

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EUR 736.67
Description: Available in various conjugation types.

APC rat IgG1, κ Isotype Control

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EUR 173.33
Description: Available in various conjugation types.

APC rat IgG1, κ Isotype Control

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EUR 368.33
Description: Available in various conjugation types.

FITC rat IgM, κ Isotype Control

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EUR 303.33
Description: Available in various conjugation types.

FITC rat IgM, κ Isotype Control

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EUR 1083.33
Description: Available in various conjugation types.

PE rat IgG2a, κ Isotype Control

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EUR 173.33
Description: Available in various conjugation types.

PE rat IgG2a, κ Isotype Control

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EUR 346.67
Description: Available in various conjugation types.

PE rat IgG2b, κ Isotype Control

E16FRCP003K-025 25 tests
EUR 195
Description: Available in various conjugation types.

PE rat IgG2b, κ Isotype Control

E16FRCP003K-025U 25 μg
EUR 151.67
Description: Available in various conjugation types.

PE rat IgG2b, κ Isotype Control

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EUR 346.67
Description: Available in various conjugation types.

PE rat IgG2b, κ Isotype Control

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EUR 325
Description: Available in various conjugation types.

PE rat IgG2c, κ Isotype Control

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EUR 281.67
Description: Available in various conjugation types.

Mouse IgM Isotype Control (PE)

E2790001 100ul
EUR 225
Description: Available in various conjugation types.

Mouse IgM Isotype Control

E2790006 100ul
EUR 225
Description: Available in various conjugation types.

Rat IgG2a Isotype Control

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Mouse IgM Isotype Control

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Human IgG Isotype Control

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Rat IgG2a Isotype Control

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Mouse IgA Isotype Control

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Human IgA Isotype Control

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Mouse IgM Isotype Control

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Rat IgG2a Isotype Control

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Mouse IgG Isotype Control

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Mouse IgG Isotype Control

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Rat IgG2b Isotype Control

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Mouse IgG Isotype Control

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Mouse IgA Isotype Control

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Rat IgG2b Isotype Control

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Rat IgG2b Isotype Control

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Mouse IgM Isotype Control

GWB-2B9642 1 mg Ask for price

Human IgG Isotype Control

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Mouse IgG Isotype Control

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Mouse IgA Isotype Control

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Rat IgG2b Isotype Control

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Rat IgG2b Isotype Control

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Rat IgG2b Isotype Control

GWB-15EFE0 0.5 mg Ask for price

Rat IgG2b Isotype Control

GWB-1671C8 0.5 mg Ask for price

Mouse IgG Isotype Control

GWB-1B0AB3 0.5 mg Ask for price

Mouse IgG Isotype Control

GWB-1BE5E5 10 mg Ask for price

Rat IgG2b Isotype Control

GWB-1D8308 0.1 mg Ask for price

Sheep IgG Isotype Control

GWB-1E6DA3 1 mg Ask for price

Mouse IgG Isotype Control

IC002-100ul 100ul
EUR 146.4

Mouse IgM Isotype Control

ICIGMF2-100 100 tests
EUR 396

Mouse IgM Isotype Control

ICIGMPU2-100 100 tests
EUR 396

Human IgA Isotype Control

GWB-8ADBA2 0.5 mg Ask for price

Rat IgG2a Isotype Control

GWB-8BC24F 0.1 mg Ask for price

At this concentration, the hemoglobin oxygen characterized by a much lower mobility of water in the hydration shell, measured as an increase in relaxation time, compared with deoxyhemoglobin. These changes indicate an increase in the viscosity of red blood cell cytosol when cells of oxygen and decreases the viscosity of the deoxygenation. Information provided by MDS on the water situation intraerythrocytic intact red blood cells reflects the interaction with all the components of the cytosol, make this measurement a strong predictor of changes in the rheological properties of red blood cells, regardless of the cause.

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