Oxidized low-density lipoprotein (oxLDLs) and oxysterols play a key role in the development of endothelial dysfunction and atherosclerosis. Loss of function of vascular endothelium negative impact vasomotion, cell growth, adhesiveness and barrier function. While for some of these disorders plausible explanation can be provided from a mechanistic standpoint, for many others, the molecular players involved are not known. Caveolae, specific plasma membrane domains, has recently emerged as a target and mediator of oxLDL-induced endothelial dysfunction (ED).
Caveolae and their protein Caveolin-1 related (Cav-1) involved in oxLDLs / LDL transcytosis, especially through the scavenger receptor class B type 1 (SR-B1). Instead, oxLDLs LDL receptor endocytosis mediated by lectin-like oxidized 1 (LOX-1), whose activities depend on intact caveolae system. In addition, LOX-1 regulates expression of Cav-1 and vice versa.
On the other hand, oxLDLs may affect the plasma membrane cholesterol content / distribution thus affecting the architecture of caveolae, Cav-1 localization and related signals. Overall, this body of evidence shows that caveolae have both active and passive role in oxLDL-induced EC dysfunction. First as a mediator of lipid uptake and transfer in the subendothelial space, and then, as the target of a change in the composition / plasma membrane lipid dynamics resulting from increased levels of circulating oxLDLs.
Getting a better understanding of how oxLDLs interacts with endothelial cells (EC) and caveolae-mediated signaling pathways modulate, leading to ED, it is important to find new targets of intervention to address atherosclerosis and related clinical entities.
Thiol-based switching mechanism of stress-sensing companion
Thiol-based redox switches evolved as post-translational mechanisms that allow for efficient regulation of individual proteins to quickly respond to sudden environmental changes. While some of the functions of proteins need to be turned off to conserve resources and avoid potentially error-prone process, the protective function becomes important and need to be activated. In this review, we focus on the mechanism of thiol-based activation of the stress-sensing chaperones.
After the exposure to stress, the companion converted into a high-affinity binding platform for ongoing proteins and cells to protect against the accumulation of protein aggregates that are potentially toxic. their chaperone activity independent of ATP, a feature that is very important in conditions of oxidative stress, in which cellular ATP levels drop and canonical ATP-dependent chaperone no longer operate.
Likewise, reductive inactivation and release substrates require recovery of ATP levels, which ensures clients with foldases protein folding ATP-dependent. We will provide an overview on the different strategies that cells evolved to rapidly increase an ATP-independent chaperone on oxidative stress and provide mechanistic insight into how stress conditions used to convert the cellular protein overflow into the holding ATP-independent chaperones.
Both lysosomal integral membrane proteins form a complex MFSD1 and GLMP tight that confer protection from both interaction partners against lysosomal proteolysis. We are here enhanced molecular interactions of the two proteins and found that GLMP luminal domain, but not the transmembrane domain or short cytosolic tail, the inherent protection and mediate interactions with MFSD1.Our data support the finding that the interaction is important for the stabilization of the complex.
These results are supplemented by observations that N-glycosylation of GLMP in general, but not the type N-glycans (high-mannose-type or complex-type) or individual N-glycan chains, it is very important for protection. We observed that the interaction of the two proteins has already begun in the endoplasmic reticulum, and quantitative dependent on one another.
Both proteins can affect otherwise their intracellular trafficking to lysosomes in addition to protection from proteolysis. Finally, we provide evidence that can form homodimers MFSD1 both in vitro and in vivo. Our data repair complex interaction between intimate partner of the lysosomal transporter and accessory subunit.

TREND-DB-atlas transcriptome-wide dynamic landscape of alternative polyadenylation
alternative polyadenylation (APA) extend deep transcriptome complexity. Disruption of the APA can interfere with biological processes, ultimately resulting in disruption destroy. The main challenge in identifying the mechanisms and consequences of APA (and its disturbances) lies in the complexity of the RNA 3 ‘end processing, which involves poorly conserved RNA motif and multi-component complex consisting of more than 50 proteins.
It is more complicated that the RNA 3 ‘end maturation closely associated with transcription, RNA processing and even epigenetic (histone / DNA / RNA) modification. Here, we present TREND-DB , resource cataloging dynamic landscape APA after depletion> 170 proteins involved in various aspects of transcription, gene regulation co- and post-transcriptional, and epigenetic modifications further processing. TREND-DB visualization transcriptome dynamics 3 ‘diversification end (TREND) in a very interactive; APA provides global network maps and allows interrogating genes influenced by APA-specific regulator and vice versa. It also allows the analysis of functional enrichment of specific conditions WHAT affected genes, which show wide biological and clinical relevance in all conditions of RNAi.
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L1033-5 | ApexBio | 5 mg/well | EUR 9005 |
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G40-300 | GeneOn | 50µg | EUR 56 |
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G40-305 | GeneOn | 5x50µg | EUR 147 |
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abx023805-02mg | Abbexa | 0.2 mg | EUR 648 |
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ABF5235 | Lifescience Market | 100 ug | EUR 438 |
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ABF9055 | Lifescience Market | 100 ug | EUR 438 |
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ABD3082 | Lifescience Market | 100 ug | EUR 438 |
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10C-CR6034M1 | Fitzgerald | 100 ug | EUR 241 |
Description: Mouse monoclonal DNA antibody |
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10-D20A | Fitzgerald | 500 ug | EUR 493 |
Description: Mouse monoclonal DNA antibody |
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10R-D114b | Fitzgerald | 100 ug | EUR 560 |
Description: Mouse monoclonal DNA antibody |
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310-005 | GeneOn | 200µg | EUR 82 |
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310-025 | GeneOn | 5x200 µg | EUR 225 |
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DF37100-1 | Neuromics | 1 ml | EUR 349 |
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DF37293-1 | Neuromics | 1 ml | EUR 291 |
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MSD14 | Bio Basic | 50ug | EUR 152.66 |
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DNAF001 | Bio Basic | 1ml | EUR 278.81 |
|
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AD-146-B | Alpha Diagnostics | Custom | Ask for price |
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AD-146-U | Alpha Diagnostics | Custom | Ask for price |
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abx257816-96tests | Abbexa | 96 tests | EUR 746 |
|
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abx232431-100ug | Abbexa | 100 ug | EUR 481 |
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N150 | GeneOn | 500 units | EUR 102 |
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N152 | GeneOn | 5x500 units | EUR 394 |
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N154 | GeneOn | 20x500 units | EUR 1525 |
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AD-146-F | Alpha Diagnostics | Custom | Ask for price |
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AD-155-B | Alpha Diagnostics | Custom | Ask for price |
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AD-155-U | Alpha Diagnostics | Custom | Ask for price |
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1-CSB-YP889065HU | Cusabio |
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Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in Yeast |
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1-CSB-EP889065HU | Cusabio |
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Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in E.coli |
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1-CSB-BP889065HU | Cusabio |
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Description: Recombinant Human DNA-directed DNA/RNA polymerase mu(POLM) expressed in Baculovirus |
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N140 | GeneOn | 500 units | EUR 50 |
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N142 | GeneOn | 5x500 units | EUR 120 |
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N144 | GeneOn | 20x500 units | EUR 367 |
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L1007-5 | ApexBio | 5 mg/well | EUR 5977 |
Description: A wide range of well-characterized bioactive molecules that covers various targets related to DNA damage/DNA repair, including ATM/ATR, HDAC, and topoisomerase etc. Facilitate your research towards the insights of cancer, genome instability and immune diseases etc. |
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P2815 | FN Test | 100ug | Ask for price |
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Description: Recombinant protein for human DNA-directed DNA/RNA polymerase mu |
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AF5340 | Affbiotech | 200ul | EUR 304 |
Description: DNA PK Antibody detects endogenous levels of total DNA PK. |
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BH40501 | Bioatlas | 1ml | EUR 103 |
Description: For DNA staining and vizualization. |
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BIO-21040 | Bioline | 500 Units | Ask for price |
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BIO-21046 | Bioline | 250 Units | Ask for price |
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BIO-21047 | Bioline | 500 Units | Ask for price |
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BIO-21048 | Bioline | 5000 Units | Ask for price |
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BIO-21051 | Bioline | 250 Units | Ask for price |
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BIO-21052 | Bioline | 500 Units | Ask for price |
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BIO-21060 | Bioline | 2500 Units | Ask for price |
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BIO-21078 | Bioline | 5000 Units | Ask for price |
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BIO-21082 | Bioline | 2000 Units | Ask for price |
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BIO-21083 | Bioline | 1000 Units | Ask for price |
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BIO-21098 | Bioline | 250 Units | Ask for price |
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BIO-21099 | Bioline | 500 Units | Ask for price |
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BIO-21105 | Bioline | 500 units | Ask for price |
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BIO-21105/S | Bioline | Sample | Ask for price |
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BIO-21106 | Bioline | 2500 units | Ask for price |
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BIO-21107 | Bioline | 5000 units | Ask for price |
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BIO-21117 | Bioline | 250 Units | Ask for price |
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BIO-21117/S | Bioline | Sample | Ask for price |
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BIO-21118 | Bioline | 500 Units | Ask for price |
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BIO-21119 | Bioline | 2500 Units | Ask for price |
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BIO-21121 | Bioline | 250 Units | Ask for price |
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BIO-21121/S | Bioline | Sample | Ask for price |
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BIO-21122 | Bioline | 500 Units | Ask for price |
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BIO-35025 | Bioline | 500µl @ 200ng/µl | Ask for price |
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BA00103 | Cusabio | 500U | EUR 87 |
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
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BA00104 | Bioatlas | 1000U | EUR 123 |
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
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BA00105 | Bioatlas | 2500U | EUR 232 |
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
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BA00203 | Bioatlas | 500U | EUR 145 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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BA00204 | Bioatlas | 1000U | EUR 239 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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BA00205 | Bioatlas | 2500U | EUR 522 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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BA00303 | Bioatlas | 500U | EUR 123 |
Description: High quality RedTaq polymerase for different PCR variations and downstream applications. |
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BA00304 | Bioatlas | 1000U | EUR 196 |
Description: High quality RedTaq polymerase for different PCR variations and downstream applications. |
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BA00305 | Bioatlas | 2500U | EUR 413 |
Description: High quality RedTaq polymerase for different PCR variations and downstream applications. |
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BA00503 | Bioatlas | 500U | EUR 145 |
Description: High quality Pfu polymerase for different PCR variations and downstream applications. |
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BA00504 | Bioatlas | 1000U | EUR 239 |
Description: High quality Pfu polymerase for different PCR variations and downstream applications. |
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BA00505 | Bioatlas | 2500U | EUR 522 |
Description: High quality Pfu polymerase for different PCR variations and downstream applications. |
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BT10101 | Bioatlas | 500U | EUR 80 |
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
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BT10102 | Bioatlas | 1000U | EUR 109 |
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
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BT10103 | Bioatlas | 2000U | EUR 167 |
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
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BT10201 | Bioatlas | 500U | EUR 120 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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BT10202 | Bioatlas | 1000U | EUR 190 |
Description: High quality HotTaq polymerase for different PCR variations and downstream applications. |
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AF0550 | Affbiotech | 200ul | EUR 304 |
Description: DNA-PK Antibody detects endogenous levels of DNA-PK. |
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HY-109517 | MedChemExpress | 100mg | EUR 1910 |
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FYG601-100P | Yeastern Biotech | 100 Preps | Ask for price |
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FYG601-300P | Yeastern Biotech | 300 Preps | Ask for price |
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G030 | ABM | 6X; 3 x 1.0 ml | EUR 79 |
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G498 | ABM | 250 U (50 ul) | EUR 87 |
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G499 | ABM | 1000 U (200 ul) | EUR 158 |
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20-abx110554 | Abbexa |
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ABP-BVD-10001 | Allele Biotech | 15 µg (10 rxns) | Ask for price |
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abx149863-100ug | Abbexa | 100 ug | EUR 439 |
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20-abx098045 | Abbexa |
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20-abx098048 | Abbexa |
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20-abx098049 | Abbexa |
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20-abx098050 | Abbexa |
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20-abx098052 | Abbexa |
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20-abx098076 | Abbexa |
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abx098136-10kU | Abbexa | 10 kU | EUR 321 |
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abx098136-20Ku | Abbexa | 20 Ku | EUR 398 |
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abx098143-5ml | Abbexa | 5 ml | EUR 175 |
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abx098870-50rxns | Abbexa | 50 rxns | EUR 398 |
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abx020668-02mg | Abbexa | 0.2 mg | EUR 523 |
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ABF5340 | Lifescience Market | 100 ug | EUR 438 |
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ABF0550 | Lifescience Market | 100 ug | EUR 438 |
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abx298027-50rxns | Abbexa | 50 rxns | EUR 398 |
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70R-50261 | Fitzgerald | 100 ul | EUR 244 |
Description: Purified Polyclonal DNA PKcs antibody |
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9001-2500 | Biovision | EUR 512 |
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9001-500 | Biovision | EUR 191 |
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9003-2500 | Biovision | EUR 512 |
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9003-500 | Biovision | EUR 180 |
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9004-2500 | Biovision | EUR 610 |
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9004-500 | Biovision | EUR 218 |
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402-002 | GeneOn | 400 WEISS UNITS | EUR 63 |
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402-010 | GeneOn | 2000 WEISS UNITS | EUR 154 |
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48923-100ul | SAB | 100ul | EUR 333 |
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48923-50ul | SAB | 50ul | EUR 239 |
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10R-10729 | Fitzgerald | 100 ug | EUR 381 |
Description: Mouse monoclonal DNA PKcs antibody |
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10-101 | Sceti | 20ug | EUR 388 |
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur. |
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10-102 | Sceti | 100ug | EUR 923 |
Description: The DNA polymerase ? (Rat) is available in Europe and for worldwide shipping via Gentaur. |
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3130-100 | Biovision | EUR 338 |
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301-025 | GeneOn | 500 µg | EUR 102 |
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301-100 | GeneOn | 5x500µg | EUR 355 |
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33358-100ul | SAB | 100ul | EUR 252 |
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33358-50ul | SAB | 50ul | EUR 187 |
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20R-1466 | Fitzgerald | 100 ug | EUR 673 |
Description: Rabbit polyclonal DNA PK antibody |
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20-ZS20 | Fitzgerald | 250 ug | EUR 421 |
Description: Sheep polyclonal Z-DNA antibody |
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20R-2017 | Fitzgerald | 50 ug | EUR 281 |
Description: Rabbit polyclonal DNA PKcs antibody |
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20R-2346 | Fitzgerald | 50 ug | EUR 281 |
Description: Rabbit polyclonal DNA PKcs antibody |
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6023 | Chondrex | 1 kit | EUR 180.5 |
Description: DNA Assay Kit |
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7-03778 | CHI Scientific | 2,000U | Ask for price |
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7-03779 | CHI Scientific | 10,000U | Ask for price |
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7-03780 | CHI Scientific | 20,000U | Ask for price |
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K1416-250 | Biovision | EUR 561 |
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K1416-50 | Biovision | EUR 316 |
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D006-100 | Cygnus Technologies | 100 ml | EUR 373 |
|
|||
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
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D006-1000 | Cygnus Technologies | 1000 ml | EUR 1167 |
|
|||
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
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D006-500 | Cygnus Technologies | 500 ml | EUR 726 |
|
|||
Description: DNA Sample Diluent by Cygnus Technologies is available in Europe via Gentaur. |
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D106 | Cygnus Technologies | 55 ml | EUR 175 |
|
|||
Description: DNA Precipitation Buffer by Cygnus Technologies is available in Europe via Gentaur. |
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D1100-050 | GenDepot | 50㎍ | EUR 165 |
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D1100-250 | GenDepot | 5X50㎍ | EUR 550 |
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D1101-050 | GenDepot | 50㎍ | EUR 131 |
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D1101-250 | GenDepot | 5X50㎍ | EUR 300 |
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D162 | Cygnus Technologies | 1 ml | EUR 506 |
|
|||
Description: Human DNA Concentrate by Cygnus Technologies is available in Europe via Gentaur. |
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D412 | Cygnus Technologies | 1 ml | EUR 542 |
|
|||
Description: E.coli DNA Concentrate by Cygnus Technologies is available in Europe via Gentaur. |
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D552 | Cygnus Technologies | 1 ml | EUR 542 |
|
|||
Description: CHO DNA Concentrate by Cygnus Technologies is available in Europe via Gentaur. |
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D556 | Cygnus Technologies | 120 ul | EUR 469 |
|
|||
Description: CHO DNA Concentrate by Cygnus Technologies is available in Europe via Gentaur. |
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N101-01 | Vazyme | 2000 U | EUR 232 |
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MD102-01 | Vazyme | 250 μl | EUR 107 |
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MD102-02 | Vazyme | 500 μl | EUR 113 |
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MD103-01 | Vazyme | 250 μl | EUR 109 |
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MD103-02 | Vazyme | 500 μl | EUR 115 |
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M1211-100 | Biovision | EUR 321 |
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M1234-1000 | Biovision | EUR 213 |
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M1239-1000 | Biovision | EUR 311 |
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M1146-1000 | Biovision | EUR 332 |
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M1146-10000 | Biovision | EUR 1066 |
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M1146-5000 | Biovision | EUR 800 |
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M1148-1000 | Biovision | EUR 1039 |
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M1148-250 | Biovision | EUR 387 |
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M1150-1000 | Biovision | EUR 686 |
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M1150-250 | Biovision | EUR 278 |
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M1151-1000 | Biovision | EUR 746 |
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M1151-250 | Biovision | EUR 311 |
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M1152-1000 | Biovision | EUR 800 |
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M1152-250 | Biovision | EUR 332 |
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L7051001 | Biochain | 1000 unit | EUR 195 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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L7051200 | Biochain | 200 unit | EUR 87 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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LB3401010 | Biochain | 10 ml | EUR 79 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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P-1006 | EpiGentek | 100 Samples | EUR 253 |
Description: Ask the seller for details |
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P401-d1 | Vazyme | 250 U | EUR 136 |
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P401-d2 | Vazyme | 1000 U | EUR 227 |
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P401-d3 | Vazyme | 3000 U | EUR 447 |
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S116 | GeneOn | 1x250 units | EUR 76 |
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S117 | GeneOn | 2x250 units | EUR 94 |
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S118 | GeneOn | 10x250 units | EUR 303 |
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S600 | GeneOn | 2000 U | EUR 107 |
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RP-1470 | Alpha Diagnostics | 2000 IU | EUR 286 |
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TG2013-1 | TopoGen | 25 ug | EUR 268 |
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TG2013-2 | TopoGen | 50 ug | EUR 380 |
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TG2013-3 | TopoGen | 250 ug | EUR 1052 |
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YF-PA24465 | Abfrontier | 50 ul | EUR 334 |
Description: Mouse polyclonal to DNA PKcs |
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Z5030023 | Biochain | 250 assays | EUR 647 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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X11000 | EpiGentek | 0.2 ml | Ask for price |
Description: reagents widely cited |
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MSD15 | Bio Basic | 50ug | EUR 122.21 |
|
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MSD16 | Bio Basic | 50ug | EUR 113.51 |
|
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MSD17 | Bio Basic | 50ug | EUR 113.51 |
|
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SB2040 | Bio Basic | 100UL, 100UL | EUR 67.4 |
|
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SK8201 | Bio Basic | 100preps | EUR 93.5 |
|
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9K-004-0002 | Bio Basic | 0.5ml | EUR 217.48 |
|
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|||
BS8202 | Bio Basic | 100preps | EUR 88.06 |
|
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|||
AD-155-F | Alpha Diagnostics | Custom | Ask for price |
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|||
ELA-E1126r | Lifescience Market | 96 Tests | EUR 886 |
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20-abx008825 | Abbexa |
|
|
|
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20-abx013061 | Abbexa |
|
|
|
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abx364935-96tests | Abbexa | 96 tests | EUR 511 |
|
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|||
abx364947-96tests | Abbexa | 96 tests | EUR 511 |
|
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|||
abx232474-100ug | Abbexa | 100 ug | EUR 509 |
|
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50 | Biobase | 96T/Box | Ask for price |
|
|||
Description: ELISA based test for quantitative detection of ds-DNA (Double-stranded DNA Antibody IgG) |
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|||
51 | Biobase | 96T/Box | Ask for price |
|
|||
Description: ELISA based test for quantitative detection of ss-DNA (Single-stranded DNA Antibody IgG) |
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|||
TG2000G-1KIT | TopoGen | 100 assays | EUR 380 |
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TG2000G-3KIT | TopoGen | 500 assays | EUR 985 |
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|||
TG2000G-5KIT | TopoGen | 1000 assays | EUR 1612 |
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|||
TG2000G-7KIT | TopoGen | 2000 assays | EUR 2844 |
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EH2936 | FN Test | 96T | EUR 524.1 |
|
|||
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
Implementation of the UCSC Genome Browser provides additional customized layer of gene regulation accounting for individual transcript isoforms (eg, epigenetic, miRNA-binding sites and RNA-binding protein). TREND-DB that encourages APA outlines roles for various biological program, including potential disease mechanisms, and help identify potential diagnostic and therapeutic them.