The Notch signaling network to determine stemness in various tissues and targeting indicates activity on malignant brain cancer by gamma-Secretase inhibitor (GSI) has shown promising preclinical success. However, the translation of the remains clinically challenging because the side effects of severe toxicity and the emergence of resistance to therapy. Better anti-Notch therapies directed, particularly directed against the tumor promoting receptor Notch 1 signaling framework, and biomarkers that predict response to therapy of the highest clinical needs.
We assessed several patient dataset to investigate the clinical relevance of activation of Notch1 and possible differential distribution between molecular subtypes in brain cancer. We functionally assess the biological effects of the first-in-man is tested blocking antibody against the receptor Notch1 (brontictuzumab, BRON) in the collection of stem-like cell model of glioma (GSC) and compared the effect of genetic inhibition of Notch1 and pharmacological classic Notch inhibitor treatment using gamma-Secretase MRK003 inhibitor.
We also assess the effect on winged (NTB) stem cell activation signal, which includes interrogation NTB models genetic inhibition. calculated transcription Notch pathway activation score we upregulated in neural stem cells, as compared to astrocytes; as well as in GSCs, compared with differentiated glioblastoma cells.
In addition, the signature Notch is a glioblastoma clinical prediction on the discovery and validation of our patient cohort. Notch signature significantly elevated in tumors with mutant genomes IDH1 and tumors without 1p and 19q co-deletion. In GSCs with increased expression of Notch1, BRON treatment block gene transcription Notch pathway target Hes1 / Hey1, significantly reducing the amount of cleaved Notch1 receptor protein and cause a significant decrease in cellular invasion. Benchmarking this phenotype to that observed with the genetic inhibition of Notch1 in the appropriate cell model did result in a higher reduction of cell invasion under chemotherapy.
BRON treatment of the signs resulting from upregulation of winged (NTB) stem cell activity signals, and vice versa, due to blockage of WNT signaling induced expression of Notch target genes in our model. We extend the list of evidence that elevated expression of Notch signaling biomarker signature stating the prevalence of stem cells and clinically useful for predicting negative in glioblastoma. By using functional tests, we first validated in human Notch1 receptor-specific antibodies were tested as promising candidates in the context of neuro-oncology and biomarker panel proposes to predict the durability and therapeutic success of these treatment options. We note that the observed phenotype appears to be partial because of Notch1 blockage and lead candidate to target signal activation off.
Further studies addressing the possible emergence of resistance to therapy because NTB need activation will be done. We further validated our 3D disease modeling technology to be a benefit for development projects.
Protein arginine methyltransferase 5 pICln promote androgen receptor-dependent transcription in castration-resistant prostate cancer
The majority of prostate cancer therapy aims to inhibit the androgen receptor (AR) signaling. However, AR reactivation certainly encourage the development of disease castration-resistant prostate cancer (CRPC). Here we show that the protein arginine methyltransferase 5 (PRMT5) serves as an epigenetic activator of transcription AR in CRPC, requires cooperation with methylosome pICln subunit. In vitro and in xenograft tumors in mice, targeting PRMT5 or pICln pressed CRPC cell growth.
Full-length AR and AR-V7 good transcriptional activation required PRMT5 and pICln but not MEP50. This activation of transcription-mediated accompanied by PRMT5 dimethylation H4R3 symmetrical in proximal promoter AR. Furthermore, knockdown of PRMT5 abolished binding pICln (but not vice versa) to the proximal promoter region AR, showed that recruiting PRMT5 plan to AR AR promoter to activate transcription. Analysis of differential gene expression in cells 22Rv1 PRMT5 and pICln confirms that both regulate androgen signaling pathways.
Additionally, PRMT5 and pICln protein expression was positively correlated with AR and AR-V7 protein expression in CRPC tissues and their expression is highly correlated to the level of mRNA in several publicly available datasets CRPC. Our results show that targeting PRMT5 or pICln can be explored as a new therapy for the treatment of CRPC by suppressing the expression of AR and AR AR splice variants to avoid reactivation.
Chimerism as a basis for improvement of organ
Organ and tissue repair is a complex process that involves signaling molecules, growth factors and cell cycle regulators acting in concert to promote cell division and differentiation at the site of injury. In embryonic development, the fetal progenitor cells are actively involved in reparations and displays biphasic interaction with the mother; and there is a constant trade fetal cells into the maternal circulation and vice versa.
This phenomenon of fetal microchimerism may have a significant impact given the primitive multilineage properties of these cells. In published work, we have reported that fetal-derived placental cells express the homeodomain protein CDX2 retain all the “stem” of embryonic stem cell functional protein is not blessed with additional functionality in the areas of growth, survival, homing, and immune modulation.
Dimethylsulfoxide (Molecular Biology Grade) |
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| CE120 | GeneOn | 100 ml | EUR 66 |
Dimethylsulfoxide (Molecular Biology Grade) |
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| CE121 | GeneOn | 500 ml | EUR 110.4 |
DTT (Molecular Biology Grade) |
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| CE131 | GeneOn | 5 g | EUR 93.6 |
DTT (Molecular Biology Grade) |
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| CE132 | GeneOn | 10 g | EUR 133.2 |
DTT (Molecular Biology Grade) |
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| CE133 | GeneOn | 25 g | EUR 243.6 |
Glycine (Molecular Biology Grade) |
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| CE158 | GeneOn | 1 kg | EUR 84 |
Glycine (Molecular Biology Grade) |
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| CE159 | GeneOn | 5 kg | EUR 228 |
HEPES (Molecular Biology Grade) |
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| CE171 | GeneOn | 100 g | EUR 98.4 |
HEPES (Molecular Biology Grade) |
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| CE172 | GeneOn | 500 g | EUR 268.8 |
HEPES (Molecular Biology Grade) |
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| CE173 | GeneOn | 1 kg | EUR 424.8 |
Lysozyme (Molecular Biology Grade) |
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| CE188 | GeneOn | 1 g | EUR 70.8 |
Lysozyme (Molecular Biology Grade) |
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| CE189 | GeneOn | 10 g | EUR 247.2 |
NAD (Molecular Biology Grade) |
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| CE196 | GeneOn | 1 g | EUR 72 |
NAD (Molecular Biology Grade) |
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| CE197 | GeneOn | 5 g | EUR 165.6 |
NBT (Molecular Biology Grade) |
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| CE209 | GeneOn | 1 g | EUR 123.6 |
NBT (Molecular Biology Grade) |
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| CE210 | GeneOn | 5 g | EUR 360 |
Tris (Molecular Biology Grade) |
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| CE237 | GeneOn | 500 g | EUR 106.8 |
Tris (Molecular Biology Grade) |
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| CE238 | GeneOn | 1 kg | EUR 153.6 |
Tris (Molecular Biology Grade) |
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| CE239 | GeneOn | 5 kg | EUR 535.2 |
Tween20 (Molecular Biology Grade) |
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| CE242 | GeneOn | 1 l | EUR 106.8 |
Water (Molecular Biology Grade) |
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| CE243 | GeneOn | 500 ml | EUR 62.4 |
Water (Molecular Biology Grade) |
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| CE244 | GeneOn | 1 l | EUR 67.2 |
100mL Molecular Biology Grade |
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| 46-000-CI | Scientific Laboratory Supplies | PK6 | EUR 70.68 |
500mL Molecular Biology Grade |
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| 46-000-CV | Scientific Laboratory Supplies | PK6 | EUR 124.26 |
Water, Ultrapure Molecular Biology Grade |
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| 41024-4L | Biotium | 4L | EUR 145.2 |
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Description: Minimum order quantity: 1 unit of 4L |
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Ammonium sulfate (Molecular Biology Grade) |
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| CE105 | GeneOn | 250 g | EUR 55.2 |
Ammonium sulfate (Molecular Biology Grade) |
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| CE106 | GeneOn | 1 kg | EUR 72 |
Ammonium sulfate (Molecular Biology Grade) |
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| CE107 | GeneOn | 5 kg | EUR 153.6 |
Bis-Acrylamid (Molecular Biology Grade) |
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| CE110 | GeneOn | 50 g | EUR 94.8 |
Bis-Acrylamid (Molecular Biology Grade) |
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| CE111 | GeneOn | 250 g | EUR 259.2 |
Formamide deionized (Molecular Biology Grade) |
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| CE145 | GeneOn | 500 ml | EUR 87.6 |
Formamide deionized (Molecular Biology Grade) |
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| CE146 | GeneOn | 1 l | EUR 120 |
Glycerol 87 % (Molecular Biology Grade) |
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| CE154 | GeneOn | 1 l | EUR 93.6 |
Glycerol waterfree (Molecular Biology Grade) |
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| CE155 | GeneOn | 500 ml | EUR 78 |
Glycerol waterfree (Molecular Biology Grade) |
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| CE156 | GeneOn | 1 l | EUR 102 |
Glycerol waterfree (Molecular Biology Grade) |
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| CE157 | GeneOn | 2.5 l | EUR 170.4 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
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| CE160 | GeneOn | 100 g | EUR 93.6 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
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| CE161 | GeneOn | 250 g | EUR 153.6 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
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| CE162 | GeneOn | 500 g | EUR 232.8 |
Guanidine - Hydrochloride (Molecular Biology Grade) |
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| CE163 | GeneOn | 1 kg | EUR 352.8 |
Guanidine Thiocyanate (Molecular Biology Grade) |
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| CE164 | GeneOn | 100 g | EUR 86.4 |
Guanidine Thiocyanate (Molecular Biology Grade) |
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| CE165 | GeneOn | 500 g | EUR 192 |
Guanidine Thiocyanate (Molecular Biology Grade) |
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| CE166 | GeneOn | 1 kg | EUR 307.2 |
Urea Crystalline (Molecular Biology Grade) |
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| CE167 | GeneOn | 1 kg | EUR 72 |
Urea Crystalline (Molecular Biology Grade) |
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| CE168 | GeneOn | 5 kg | EUR 181.2 |
MOPS buffer (Molecular Biology Grade) |
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| CE194 | GeneOn | 100 g | EUR 102 |
MOPS buffer (Molecular Biology Grade) |
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| CE195 | GeneOn | 250 g | EUR 169.2 |
Sodium chloride (Molecular Biology Grade) |
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| CE205 | GeneOn | 500 g | EUR 62.4 |
Sodium chloride (Molecular Biology Grade) |
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| CE206 | GeneOn | 1 kg | EUR 70.8 |
Sodium chloride (Molecular Biology Grade) |
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| CE207 | GeneOn | 5 kg | EUR 123.6 |
D(+)-Sucrose (Molecular Biology Grade) |
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| CE224 | GeneOn | 500 g | EUR 67.2 |
D(+)-Sucrose (Molecular Biology Grade) |
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| CE225 | GeneOn | 1 kg | EUR 84 |
D(+)-Sucrose (Molecular Biology Grade) |
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| CE226 | GeneOn | 5 kg | EUR 207.6 |
Tris - Hydrochloride (Molecular Biology Grade) |
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| CE234 | GeneOn | 250 g | EUR 99.6 |
Tris - Hydrochloride (Molecular Biology Grade) |
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| CE235 | GeneOn | 500 g | EUR 144 |
Tris - Hydrochloride (Molecular Biology Grade) |
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| CE236 | GeneOn | 1 kg | EUR 223.2 |
TritonX-100 (Molecular Biology Grade) |
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| CE240 | GeneOn | 500 ml | EUR 67.2 |
TritonX-100 (Molecular Biology Grade) |
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| CE241 | GeneOn | 1 l | EUR 79.2 |
Urea, suitable for molecular biology |
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| GE1210-1KG | Glentham Life Sciences | 1 kg | EUR 106.8 |
Urea, suitable for molecular biology |
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| GE1210-500G | Glentham Life Sciences | 500 g | EUR 76.8 |
Tween 20, Molecular Biology Grade |
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| T9100-010 | GenDepot | 100ml | EUR 86.4 |
Tween 20, Molecular Biology Grade |
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| T9100-050 | GenDepot | 500ml | EUR 133.2 |
Tween 20, Molecular Biology Grade |
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| T9100-100 | GenDepot | 1L | EUR 160.8 |
1L Molecular Biology Grade Water |
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| 46-000-CM | Scientific Laboratory Supplies | PK6 | EUR 177.84 |
EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade) |
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| CE135 | GeneOn | 250 g | EUR 72 |
EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade) |
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| CE136 | GeneOn | 500 g | EUR 86.4 |
EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade) |
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| CE137 | GeneOn | 1 kg | EUR 124.8 |
EDTA - Dinatriumsalz - Dihydrat (Molecular Biology Grade) |
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| CE138 | GeneOn | 5 kg | EUR 418.8 |
D(+)-Glucose waterfree (Molecular Biology Grade) |
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| CE148 | GeneOn | 500 g | EUR 67.2 |
D(+)-Glucose waterfree (Molecular Biology Grade) |
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| CE149 | GeneOn | 1 kg | EUR 75.6 |
D(+)-Glucose waterfree (Molecular Biology Grade) |
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| CE150 | GeneOn | 5 kg | EUR 180 |
Yeast extract powder (Molecular Biology Grade) |
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| CE169 | GeneOn | 500 g | EUR 133.2 |
Hyaluronidase Grade I (Molecular Biology Grade) |
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| CE174 | GeneOn | 1 g | EUR 232.8 |
Hyaluronidase Grade I (Molecular Biology Grade) |
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| CE175 | GeneOn | 5 g | EUR 920.4 |
Magnesium acetate - Tetrahydrate (Molecular Biology Grade) |
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| CE190 | GeneOn | 500 g | EUR 98.4 |
NADH - Disodium salt (Molecular Biology Grade) |
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| CE198 | GeneOn | 1 g | EUR 91.2 |
NADH - Disodium salt (Molecular Biology Grade) |
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| CE199 | GeneOn | 5 g | EUR 244.8 |
NADP - sodium salt (Molecular Biology Grade) |
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| CE200 | GeneOn | 250 mg | EUR 92.4 |
NADP - sodium salt (Molecular Biology Grade) |
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| CE201 | GeneOn | 1 g | EUR 190.8 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
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| CE202 | GeneOn | 25 mg | EUR 70.8 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
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| CE203 | GeneOn | 100 mg | EUR 126 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
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| CE204 | GeneOn | 500 mg | EUR 374.4 |
SSC Buffer (20X) (Molecular Biology Grade) |
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| CE229 | GeneOn | 1 l | EUR 86.4 |
XTT sodium salt (Molecular Biology Grade) |
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| CE250 | GeneOn | 100 mg | EUR 208.8 |
XTT sodium salt (Molecular Biology Grade) |
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| CE251 | GeneOn | 500 mg | EUR 612 |
DiscoveryProbe? Cancer Biology-related Compounds Panel |
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| L1003-5 | ApexBio | 5 mg/well | EUR 4736.4 |
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Description: A wide range of well-characterized bioactive molecules that covers various targets related to cancer biology, including p53, EGFR and PKC etc. Facilitate your research towards the insights of gene regulation, tumorgenesis and immunotherapy etc. |
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Sucrose, GlenBiol, suitable for molecular biology |
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| GC3201-1KG | Glentham Life Sciences | 1 kg | EUR 90 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
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| CE100 | GeneOn | 50 g | EUR 128.4 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
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| CE101 | GeneOn | 100 g | EUR 193.2 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
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| CE102 | GeneOn | 250 g | EUR 387.6 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
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| CE103 | GeneOn | 500 g | EUR 656.4 |
Albumin fraction V (pH7,0) (Molecular Biology Grade) |
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| CE104 | GeneOn | 1 kg | EUR 1162.8 |
Water, Ultra Pure, Sterile Molecular Biology Grade |
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| 18-193 | Genesee Scientific | 100ml/Unit | EUR 268 |
Water, Ultra Pure, Sterile Molecular Biology Grade |
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| 18-194 | Genesee Scientific | 500ml/Unit | EUR 294 |
Water, Ultra Pure, Sterile Molecular Biology Grade |
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| 18-195 | Genesee Scientific | 1000ml/Unit | EUR 308 |
Water, Ultra Pure, Sterile Molecular Biology Grade |
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| 18-196 | Genesee Scientific | 4 x 1L/Unit | EUR 471 |
Dendritic Cells/B Cells Antibody |
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| abx412775-02mg | Abbexa | 0.2 mg | EUR 678 |
anti-CD45 RA B-cells, T-cells, NK-cells |
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| 516-A-01mg | Virogen | 0,1 mg | EUR 321 |
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Description: anti-CD45 RA B-cells, T-cells, NK-cells |
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anti-CD45 RA B-cells, T-cells, NK-cells |
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| 516-A-1000ug | Virogen | 1000 ug | EUR 1539 |
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Description: anti-CD45 RA B-cells, T-cells, NK-cells |
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5637 cells |
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| C0002001 | Addexbio | One Frozen vial | EUR 582 |
MB49 cells |
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| C0002004 | Addexbio | One Frozen vial | EUR 932.4 |
K562 cells |
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| C0003004 | Addexbio | One Frozen vial | EUR 546 |
U937 cells |
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| C0003005 | Addexbio | One Frozen vial | EUR 546 |
Raji cells |
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| C0003009 | Addexbio | One Frozen vial | EUR 546 |
Daudi cells |
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| C0003010 | Addexbio | One Frozen vial | EUR 546 |
RS4;11 cells |
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| C0003012 | Addexbio | One Frozen vial | EUR 546 |
NAMALWA cells |
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| C0003013 | Addexbio | One Frozen vial | EUR 546 |
BJAB cells |
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| C0003016 | Addexbio | One Frozen vial | EUR 651.6 |
FO cells |
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| C0003017 | Addexbio | One Frozen vial | EUR 582 |
MC116 cells |
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| C0003021 | Addexbio | One Frozen vial | EUR 582 |
P3X63Ag8.653 cells |
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| C0003028 | Addexbio | One Frozen vial | EUR 546 |
REH cells |
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| C0003031 | Addexbio | One Frozen vial | EUR 582 |
Mino cells |
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| C0003033 | Addexbio | One Frozen vial | EUR 582 |
U87MG cells |
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| C0005002 | Addexbio | One Frozen vial | EUR 546 |
B103 cells |
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| C0005003 | Addexbio | One Frozen vial | EUR 932.4 |
T47D cells |
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| C0006001 | Addexbio | One Frozen vial | EUR 546 |
4T1 cells |
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| C0006004 | Addexbio | One Frozen vial | EUR 546 |
HeLa cells |
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| C0008001 | Addexbio | One Frozen vial | EUR 546 |
SiHa cells |
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| C0008002 | Addexbio | One Frozen vial | EUR 546 |
C33A cells |
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| C0008003 | Addexbio | One Frozen vial | EUR 546 |
CaSKi cells |
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| C0008004 | Addexbio | One Frozen vial | EUR 546 |
SW480 cells |
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| C0009001 | Addexbio | One Frozen vial | EUR 546 |
SW620 cells |
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| C0009002 | Addexbio | One Frozen vial | EUR 546 |
T84 cells |
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| C0009008 | Addexbio | One Frozen vial | EUR 546 |
HCT15 cells |
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| C0009010 | Addexbio | One Frozen vial | EUR 546 |
LoVo cells |
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| C0009011 | Addexbio | One Frozen vial | EUR 546 |
RKO cells |
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| C0009012 | Addexbio | One Frozen vial | EUR 546 |
SW48 cells |
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| C0009014 | Addexbio | One Frozen vial | EUR 546 |
SW1116 cells |
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| C0009019 | Addexbio | One Frozen vial | EUR 546 |
H716 cells |
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| C0009022 | Addexbio | One Frozen vial | EUR 651.6 |
OE33 cells |
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| C0013003 | Addexbio | One Frozen vial | EUR 792 |
JROECL33 cells |
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| C0013004 | Addexbio | One Frozen vial | EUR 792 |
Hep3B cells |
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| C0015001 | Addexbio | One Frozen vial | EUR 546 |
HepG2 cells |
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| C0015002 | Addexbio | One Frozen vial | EUR 546 |
Calu3 cells |
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| C0016001 | Addexbio | One Frozen vial | EUR 546 |
A549 cells |
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| C0016002 | Addexbio | One Frozen vial | EUR 546 |
H460 cells |
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| C0016003 | Addexbio | One Frozen vial | EUR 546 |
A427 cells |
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| C0016004 | Addexbio | One Frozen vial | EUR 546 |
H23 cells |
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| C0016005 | Addexbio | One Frozen vial | EUR 546 |
H1703 cells |
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| C0016008 | Addexbio | One Frozen vial | EUR 651.6 |
SW1573 cells |
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| C0016009 | Addexbio | One Frozen vial | EUR 651.6 |
H520 cells |
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| C0016010 | Addexbio | One Frozen vial | EUR 546 |
H226 cells |
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| C0016011 | Addexbio | One Frozen vial | EUR 546 |
HCC4006 cells |
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| C0016012 | Addexbio | One Frozen vial | EUR 546 |
H1975 cells |
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| C0016013 | Addexbio | One Frozen vial | EUR 651.6 |
H1650 cells |
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| C0016015 | Addexbio | One Frozen vial | EUR 651.6 |
H358 cells |
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| C0016016 | Addexbio | One Frozen vial | EUR 546 |
H2228 cells |
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| C0016017 | Addexbio | One Frozen vial | EUR 651.6 |
H2170 cells |
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| C0016018 | Addexbio | One Frozen vial | EUR 546 |
H441 cells |
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| C0016019 | Addexbio | One Frozen vial | EUR 546 |
H596 cells |
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| C0016020 | Addexbio | One Frozen vial | EUR 546 |
H1581 cells |
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| C0016028 | Addexbio | One Frozen vial | EUR 651.6 |
H2444 cells |
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| C0016038 | Addexbio | One Frozen vial | EUR 651.6 |
H69AR cells |
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| C0016046 | Addexbio | One Frozen vial | EUR 651.6 |
These cells showed multipotency in vitro and in vivo, causing spontaneous beating cardiomyocytes and blood vessel cells. In the mouse model, cells from the placenta of women CDX2 can be administered intravenously to male rats myocardial infarction with subsequent homing of cells to the area of infarction CDX2 and evidence of cell regeneration with improved cardiac function.