Technology

Label-free detection of biomolecular interactions such as drug-target interac- tions. Universal assay system ranging from small molecules up to cell based as- says. All transparent transducer materials such as glass or plastic. All standard disposables such as microscope slides or microtiter plates. Robust and flexible surface chemistry for Specific Affinity Surfaces (SAS). No thermal stabilization necessary. Extremely robust, sensitive and low costs-per test.

German sensor technology with guaranteed proof of technology for high through- put screening and high-content screening. 15 Years of experience and highly scientific reputation. Perfect for drug development, molecular diagnostics research, and basic research in life science. Low investment costs because of simple and free scalable hardware. Cheap disposables adaptable to a great variety of different assays. Built in quality control system. Added value due to a deeper insight in the underlying biochemical interactions.

• The SAS sensor element:
  Since surface affinity has the highest sensitivity in order to attract and bind specific bio-molecules selectively, the proposed technology uses Specific Affinity Surfaces (SAS) with high selectivity related to the specific target molecule in order to change the optical properties in case of an affinity reaction.
• The SAS sensor signal:
  The changed optical properties of the Specific Affinity Surface (SAS) results in a change of the reflected light intensity, which is reflected by the Specific Affinity Surface.
• Range of samples for SAS Analysis:
  The biological specimen can be blood, body fluid or other kinds of proteomic samples like cell culture media.
• Optical equipment for SAS Technology:
  The proposed technology preferably works with LED light sources under regular and almost perpendicular input angles. The analysis of the reflected light output is performed by photosensitive elements such as photo diodes or CCD cameras.
• Analyzing the SAS signals:
  The intensity of the reflected light depends just on the optical thickness of the Specific Affinity Surface. And this SAS thickness is directly depending on the concentration respectively affinity of the specific protein.
• SAS Array Sensors:
  In order to detect multiple different specific proteins respectively bio-molecules, the Specific Affinity Surface is designed as an array of (n x m) SAS Spots.

Performance of the Biametrics Technology



Experimental data:

• A Biametrics Laboratory Sensor for one SAS spot / channel is already tested Blood Group Analysis works with SAS Spots based on antibodies.
• A Biametrics Laboratory Setup for analysis of up to 1000 SAS spots on a chip is available. SAS signals show high precision and high sensitivity, up to 720 spots measured on one chip, standard deviation about 3% only. Very low noise.
• A Biametrics Prototype for High Throughput Screening is available.

Performance characteristics:

• Sensitivity and detection limit of the sensor: below 1 pg/mm2. In order to receive a highly selective signal, a LOD of the specific protein of about 1 pg/mm2 is sufficient.
• Type of samples: time resolved investigation of any kind of biomolecular interactions including whole cell assays.
• Consumables: gass and plastic consumables (transducers).
• Temperature sensitivity: highly tolerant against temperature changes. No thermostatic environment required.