Single colour reflectometry – short SCORE – can be used to analyse the binding characteristics of hundreds to thousands biomolecular interactions without the need for labels. In the past years, label-free kinetic analysis used to be available only for a few lead candidates at the end of a pharmaceutical development process. But with a high throughput technology like SCORE it now is affordable to screen a high number of candidates at the beginning of the development of therapeutic antibodies. … read more
Which hopes and fears are associated with the term “personalised medicine” today?
We recently published an article in European Biopharmaceutical Review which discusses the topic of personalised medicine in the age of next generation DNA sequencing and whole genome analysis.
In part one of his article Prof. Günter Roth demonstrated how “blind believe” in established methods for kinetic analysis might be misplaced trust. In this second part he explains how label-free imaging technology literally allows us a fresh look on binding kinetics.
Label-free analysis has made its way in the life sciences and has become a valued tool e.g. in pharmaceutical research. This is mainly due to its outstanding depth of information in combination with its reliable data quality. Yet, many other areas of application might benefit from certain advantages of label-free methods that remained largely unexploited so far. … read more
A fast and reliable characterisation of protein-protein interactions is essential for various applications in both academic research as well as the chemical and pharmaceutical industry. Label-free technologies do not require a labelling with a reporter substance and enable a real-time monitoring and the determination of kinetic parameters. Many label-free biosensor systems however suffer from a low throughput read-out. … read more
Dr. Günter Roth from the University of Freiburg presented his latest results on “Label-free real-time imaging and detection of cell-free protein microarray synthesis and interaction analysis in an integrated microfluidic system” at BITE 2017. … read more
Characterisation of cell surface antigens and their interaction partners is one of the hot topics in scientific research and development. The commonly applied biochemical approaches have certain limitations which might be overcome by label-free technologies. … read more
A fast but reliable identification of pathogens causing acute infections is a highly important issue in microbiological diagnostics. Conventional culture-based methods are well adapted and quite inexpensive but time-consuming and accompanied by certain limitations. Modern molecular methods have been increasingly incorporated in laboratories, but can they fully replace the traditional microbiological diagnostics? This article will focus on bacterial identification and characterisation methods in microbiological diagnostics including currently used technologies with their advantages and drawbacks. An overview table of the article will summarise all important information. … read more
Label-free detection technologies may suffer from non-specific binding of irrelevant proteins within the sample. Functionalisation of the sensor surface with specific (bio)polymers can inhibit this non-specific binding to a minimum. … read more
We proudly announce that the iGEM team we supported in the last year, has published an article in the current issue of BIOspektrum. Find out more about cell free protein synthesis.