Protein/Protein Interactions

Protein/Protein interactions are the basis of life to function. Explore and understand them on a molecular level and without disturbing these processes by labelling.

Epitope binning for antibodies/fab fragments

Via epitope binning, different antibodies against a similar target are tested against all other antibodies within a library in a competitive fashion. Thus, it is possible to identify antibodies that block another‘s binding to the same epitope. Using Biametrics’ SCORE technology you are able to characterise your antibody library in a smart and fast way, saving not only working time but also precious antigen material.

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HTS epitope mapping for antibody characterisation.

An important characteristic of engineered antibodies – besides their affinity – is their specificity. Especially when selecting Antibody pairs e.g. for Sandwich assays, it is important to make sure that the two recognize different epitopes.
We present a HTS solution for epitope mapping on the example of anti-Flag utilizing peptide arrays.

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Identification of (cancer) biomarkers and potential new drugs via HTS screening of protein-peptide interactions.

Protein/protein interactions play a major role of signal transduction within the cell. When the protein/protein interaction is disturbed, the signal transduction may also be disturbed and severe disease such as cancer might be the result. On the other hand, it is possible by designing peptide-based pharmaceuticals to mimic this interaction and effectively block signal transduction. This was demonstrated by us using a high density peptide array and monitoring the binding of Grb2 to this array.

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Discrimination between agonistic and antagonistic behaviour of pharmaceutical relevant substances.

Pharmaceuticals can be used to either activate or block the Estrogen Receptor Alpha. We present an elegant method to discriminate between agonistic or antagonistic behaviour of these substances by different binding behaviour of the Estrogen Receptor Alpha towards a peptide-modified transducer surface.

Diagnosis of anti-phospholipid syndrome (APS) during pregnancy.

When protein/protein interaction is disturbed, a magnitude of diseases can arise. We demonstrate how an antigen array can be used to monitor the binding of different auto-antibodies, which are involved in the development of the antiphospholipid syndrome. The applicability has been demonstrated its performance using real patient samples.

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Kinetic evaluation of PSA/anti-PSA interaction and benchmark versus other established label-free technologies.

When characterising protein/protein interactions, kinetic interaction can provide detailed insight. In this study, we show how reliable this data can be obtained using Biametrics devices and how the obtained data compares to another label-free method.

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Identification and characterisation of new cancer biomarkers.

Protein/protein interaction is involved in many crucial process within the living cell. Understanding these interactions is the key the development of new biomarkers, which can either serve a diagnostic purpose or represent a key target for therapeutic approaches. One interesting marker is represented by beta-2-microglobulin, which was detected in a direct test format.

A direct test for inflammatory biomarkers.

C-reactive Protein (CRP) represents one of the most common marker for inflammation. The levels can range between 1 and several hundreds of mg/mL under an acute inflammation. We present an assay, which is capable of monitoring CRP-levels in a wide dynamic range in a direct test format.