Understanding kinetics will give you a unique understanding of how (bio-)molecular interactions function. Knowing kinetics will enable you to characterise and compare your (bio-)molecular interactions of interest qualitatively and quantitatively and provide you deeper knowledge about the underlying principles.
Benchmark of SCORE technology versus established label-free technologies: prostate-specific antigen (PSA) / anti-PSA kinetics
Although it is possible to obtain kinetic and thermodynamic data with a multitude of different methods, some of them are better suited than others and the obtained data is not always comparable. Therefore, a well characterised antigen/antibody system was investigated using the Biametrics technology and the results were compared to another well-established label-free method.Download PDF
High-throughput kinetic studies
With Biametrics SCORE technology, it is possible to determine parameters like on/off rates, affinity constants and thermodynamic parameters of a large number of interactions in one run. Samples commonly contained in multiple micro titer plates can be immobilised and measured in parallel. Replicates, dilution and internal controls can be added to the experiment without increase of time.Download PDF
Exploring binding processes of Estrogen Receptor alpha to small molecules.
Characterising the binding mechanisms between small molecules and proteins is not trivial, even with known structures of both interaction partners. By investigating the kinetics of the Estrogen Receptor alpha towards small molecules, a deeper understanding and characterisation of the biomolecular interaction is possible.
Determining and tuning peptide affinities in HTS screening.
A HTS platform for the parallel determination of kinetic constants of hundreds of peptides towards their target protein has been set up. The high-throughput allows to speed up the process of tuning and refining peptides sequences to generate peptides with the desired affinity.Download PDF
Monitoring of hybridisation and melting processes of DNA/DNA interactions.
In classical DNA experiments, the melting point of a DNA sequence is closely related to the affinity between the two complementary sequences. Due to the temperature stability of the detection technology, it is not only possible to determine the kinetics constants and affinity, but also monitor the melting process.